Ali A
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Biochem Mol Biol Int. 1998 Jul;45(3):511-8. doi: 10.1080/15216549800202892.
Binding of naturally occurring human anti-DNA autoantibodies to beta-estradiol and native DNA has been investigated. Sera from seven SLE patients were tested for their reactivity with beta-estradiol and native calf thymus DNA. Solid Phase enzyme immunoassay was performed using both colorigenic and fluorogenic substrates. The results show enhanced binding of human anti-DNA autoantibodies to beta-estradiol as compared to native DNA. This was further substantiated by increased binding of affinity purified SLE IgG to beta-estradiol. These results suggest a role for female sex hormone in the etiopathogenesis of SLE and are significant vis-a-vis female predominance of SLE.
对天然存在的人类抗DNA自身抗体与β-雌二醇和天然DNA的结合进行了研究。检测了7名系统性红斑狼疮（SLE）患者血清与β-雌二醇和天然小牛胸腺DNA的反应性。使用显色和荧光底物进行固相酶免疫测定。结果显示，与天然DNA相比，人类抗DNA自身抗体与β-雌二醇的结合增强。亲和纯化的SLE IgG与β-雌二醇结合增加进一步证实了这一点。这些结果表明女性性激素在SLE发病机制中起作用，并且相对于SLE女性占优势而言具有重要意义。
