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由拟南芥热休克基因启动子和β-葡萄糖醛酸酶细菌基因组成的融合基因在酵母中的表达。

Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from Arabidopsis and a bacterial gene for beta-glucuronidase.

作者信息

Takahashi T, Yabe N, Komeda Y

机构信息

Molecular Genetics Research Laboratory, University of Tokyo, Japan.

出版信息

Plant Cell Physiol. 1993 Jan;34(1):161-4.

PMID:8025819
Abstract

Production of a functional beta-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

摘要

在将来自拟南芥的热激基因HSP18.2启动子控制下的GUS基因转化后,对数生长期的酵母细胞经热激处理可诱导产生功能性β-葡萄糖醛酸酶(GUS)蛋白。酵母cyr和bcy突变似乎基本没有影响。

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