Takahashi T, Yabe N, Komeda Y
Molecular Genetics Research Laboratory, University of Tokyo, Japan.
Plant Cell Physiol. 1993 Jan;34(1):161-4.
Production of a functional beta-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.
在将来自拟南芥的热激基因HSP18.2启动子控制下的GUS基因转化后,对数生长期的酵母细胞经热激处理可诱导产生功能性β-葡萄糖醛酸酶(GUS)蛋白。酵母cyr和bcy突变似乎基本没有影响。