Tsukaya H, Takahashi T, Naito S, Komeda Y
University of Tokyo, Molecular Genetics Research Laboratory, Japan.
Mol Gen Genet. 1993 Feb;237(1-2):26-32. doi: 10.1007/BF00282780.
Organ-specific and constitutive expression of the Arabidopsis HSP18.2 gene under normal growth conditions (22 degrees C) was observed in transgenic A. thaliana, which carried a fusion gene composed of the promoter region of HSP18.2, one of the genes for low molecular weight heat-shock proteins in Arabidopsis, and the gene for beta-glucuronidase (GUS) from Escherichia coli. In order to clarify the organ-specific nature of promoter expression, various mutations that affect flower morphology were introduced into the transgenic Arabidopsis line, AHS9. The results show that the pattern of expression observed in sepals, filaments, and styles is regulated in a structure-dependent manner, and suggest that the HSP18.2 gene might have an important role in the process of differentiation of flower buds, as do several other stress-related genes.
在正常生长条件（22摄氏度）下，在携带由拟南芥低分子量热休克蛋白基因之一HSP18.2的启动子区域与来自大肠杆菌的β-葡萄糖醛酸酶（GUS）基因组成的融合基因的转基因拟南芥中，观察到了拟南芥HSP18.2基因的器官特异性和组成型表达。为了阐明启动子表达的器官特异性性质，将各种影响花形态的突变引入转基因拟南芥系AHS9中。结果表明，在萼片、花丝和花柱中观察到的表达模式是以结构依赖的方式调控的，这表明HSP18.2基因可能在花芽分化过程中具有重要作用，其他几个与胁迫相关的基因也是如此。
