Weinmann W, Parker C E, Baumeister K, Maier C, Tomer K B, Przybylski M
Fakultät für Chemie, Universität Konstanz, Germany.
Electrophoresis. 1994 Feb;15(2):228-33. doi: 10.1002/elps.1150150139.
Capillary electrophoresis (CE) conditions have been developed for the separation of hydrophobic polypeptides, such as fatty acid-acylated peptides, and their subsequent structural identification by 252Cf plasma desorption (PDMS) and electrospray mass spectrometry (ESMS). Salt- and detergent-free aqueous acetic acid buffers containing up to 20% 2-propanol or 25% acetonitrile were employed for CE separations of hydrophobic peptides with (i) untreated, and (ii) 3-aminopropyltrimethoxysilane-derived fused silica capillaries. For both capillary types, electroosmotic flow rates suitable for sample isolation and transfer were determined, and CE separations of polypeptide mixtures were compared for aqueous buffers containing 2-propanol or acetonitrile. For the mass spectrometric identification of CE-separated peptides, a sheath flow sample isolation method was developed for subsequent transfer to PDMS. This procedure enabled the efficient isolation of peptide fractions for PDMS analysis, or alternative microanalytical techniques.
已经开发出毛细管电泳（CE）条件，用于分离疏水性多肽，如脂肪酸酰化肽，并随后通过252Cf等离子体解吸（PDMS）和电喷雾质谱（ESMS）对其进行结构鉴定。含有高达20%异丙醇或25%乙腈的无盐和无洗涤剂的乙酸水溶液缓冲液用于CE分离疏水性肽，使用（i）未处理的和（ii）3-氨丙基三甲氧基硅烷衍生的熔融石英毛细管。对于这两种毛细管类型，确定了适用于样品分离和转移的电渗流速率，并比较了含有异丙醇或乙腈的水性缓冲液对多肽混合物的CE分离。为了对CE分离的肽进行质谱鉴定，开发了一种鞘流样品分离方法，用于随后转移到PDMS。该程序能够有效地分离肽级分以进行PDMS分析或替代的微分析技术。
