Latthe M, Terry L, MacDonald T T
Department of Paediatric Gastroenterology, St Bartholomews Hospital, London, GB.
Eur J Immunol. 1994 Jul;24(7):1703-5. doi: 10.1002/eji.1830240737.
Immunohistochemistry on frozen sections was used to identify CD8 alpha alpha cells and CD8 alpha beta cells in human intestine. As observed previously, CD8 alpha beta cells predominate (> 95%) in tonsil and post-natal intestine. However in human fetal intestine (16-24 weeks gestation), almost half the CD8+ cells in the lamina propria are CD8 alpha alpha, and many CD8 alpha alpha cells can be identified in the epithelium. In contrast, in the T cell zones of the Peyer's patches, CD8 alpha beta cells are dominant. The CD8 alpha alpha cells are virtually all alpha beta T cell receptor positive. By analogy with the murine system, these CD8 alpha alpha cells in the fetal gut may be directly derived from the marrow, undergoing thymus-independent differentiation in the gut mucosa.
采用冷冻切片免疫组织化学方法鉴定人肠道中的CD8αα细胞和CD8αβ细胞。如先前观察到的，CD8αβ细胞在扁桃体和出生后的肠道中占主导地位（>95%）。然而，在人类胎儿肠道（妊娠16 - 24周）中，固有层中几乎一半的CD8 +细胞是CD8αα细胞，并且在上皮中可以鉴定出许多CD8αα细胞。相比之下，在派尔集合淋巴结的T细胞区，CD8αβ细胞占主导地位。CD8αα细胞几乎都是αβT细胞受体阳性。类比小鼠系统，胎儿肠道中的这些CD8αα细胞可能直接来源于骨髓，在肠道黏膜中进行非胸腺依赖的分化。
