Strzałka K, Tsugeki R, Nishimura M
Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 1994;32(1):45-9.
Heat shock proteins of hsp70 family with molecular weight of 76 and 73 kDa were detected both in etiolated as well as in greening pumpkin seedlings using immunoblotting technique. Both proteins exhibited heat shock inducibility and their apparent amount depended on the plant organ examined as well as on the greening time. Sucrose density gradient centrifugation revealed that the 76 kDa band should be attributed to chloroplasts and the 73 kDa band to mitochondrial fraction. The 76 kDa band dominated in cotyledons and was hardly detectable in root preparation whereas the 73 kDa band was prevailing in hook samples but was also clearly detectable both in cotyledons and in roots.
采用免疫印迹技术在黄化及绿化的南瓜幼苗中均检测到分子量为76 kDa和73 kDa的hsp70家族热休克蛋白。这两种蛋白均表现出热休克诱导性，其表观含量取决于所检测的植物器官以及绿化时间。蔗糖密度梯度离心显示，76 kDa条带应归因于叶绿体，73 kDa条带归因于线粒体部分。76 kDa条带在子叶中占主导，在根提取物中几乎检测不到，而73 kDa条带在弯钩样品中占优势，但在子叶和根中也能清晰检测到。
