Neumeier D, Knedel M, Würzburg U, Hennrich N, Lang H
Klin Wochenschr. 1975 Apr 1;53(7):329-33. doi: 10.1007/BF01469060.
The immunological method of determining creatine phosphokinase-MB in the serum of patients with myocardial infarction described here is based on the differential measurements of CK-activities before and after a specific immuno-precipitation of the CK-MB type. The minimum activity of the CK-MB-type which it is possible to determine with this method is 4% of the total activity. In patients with clinically confirmed myocardial infarctions 1-15% (mean 7.8%) of the total activity can be calculated as CK-activity of the MB-type on the first/second day(s) after the infarction. In patients with increased total CK-activity and without verified infarction the CK-MB content does not differ significantly from zero. The differences between the two groups are statistically significant. In patients with myocardial reinfarction the CK-MB-activity is higher than that after the first infarction. The immunological method to determine creatine-phosphokinase isoenzyme MB is of differential-diagnostic value in myocardial infarction.
本文所述的用于测定心肌梗死患者血清中肌酸磷酸激酶-MB的免疫学方法，是基于对CK-MB型进行特异性免疫沉淀前后CK活性的差异测量。用该方法能够测定的CK-MB型的最低活性为总活性的4%。在临床确诊为心肌梗死的患者中，梗死发生后的第一天/第二天，总活性的1-15%（平均7.8%）可计算为MB型的CK活性。在总CK活性升高且未证实有梗死的患者中，CK-MB含量与零无显著差异。两组之间的差异具有统计学意义。在心肌再梗死患者中，CK-MB活性高于首次梗死后的活性。测定肌酸磷酸激酶同工酶MB的免疫学方法在心肌梗死中具有鉴别诊断价值。
