[应用快速免疫碱性磷酸酶对儿童急性淋巴细胞白血病细胞进行单克隆免疫表型分析]

[Monoclonal immunophenotyping of acute lymphoblastic leukemia cells in children using rapid immune alkaline phosphatase].

作者信息

Kolenkova G V, Luk'ianova A G, Lenskaia R V, Kisliak N S, Kenny M, Makhartykh T Zh, Kondratchik K L, Chernov V M

出版信息

Gematol Transfuziol. 1994 Mar-Apr;39(2):18-21.

Abstract

Monoclonal immunophenotyping of leukemia cells of the bone marrow was carried out by the method of rapid immune alkaline phosphatase (RIAP) in 30 patients with acute lymphoblastic leukemia (ALL) aged 6 months-14 years. The authors used a panel of monoclonal antibodies (MCA) produced by Leu (Belgium) and DAKO (Denmark) directed to antigens of differentiation clusters: Tdt, HLA-DR, CD: 10, 19, 20, 22, 7, 8, 2, 5, 13, 33, 14. The results indicate diversity of compositions of differentiating antigens on leukemia cells of the dominant population and a different degree of leukemic cell pool heterogenicity. RIAP advantages over flow cytometry in immunotyping of ALL cells are shown. Arguments are provided in favor of introduction of broader MCA panel.

摘要

采用快速免疫碱性磷酸酶（RIAP）法对30例年龄在6个月至14岁的急性淋巴细胞白血病（ALL）患者的骨髓白血病细胞进行单克隆免疫表型分析。作者使用了一组由比利时的Leu和丹麦的DAKO生产的单克隆抗体（MCA），这些抗体针对分化簇抗原：末端脱氧核苷酸转移酶（Tdt）、人类白细胞抗原-DR（HLA-DR）、分化簇（CD）：10、19、20、22、7、8、2、5、13、33、14。结果表明，优势群体白血病细胞上分化抗原的组成具有多样性，白血病细胞池的异质性程度也不同。显示了RIAP在ALL细胞免疫分型中优于流式细胞术。文中提供了支持引入更广泛MCA组的论据。