Cloning and sequencing of the gene encoding benzaldehyde lyase from Pseudomonas fluorescens biovar I.
作者信息
Hinrichsen P, Gómez I, Vicuña R
机构信息
Laboratorio de Bioquímica, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago.
出版信息
Gene. 1994 Jun 24;144(1):137-8. doi: 10.1016/0378-1119(94)90218-6.
PMID:8026749
Abstract
The gene (bzl) encoding benzaldehyde lyase (BL) from Pseudomonas fluorescens biovar I has been cloned and characterized. The nucleotide sequence contains an open reading frame encoding a protein of 563 amino acids. The deduced BL protein shares little homology with proteins contained in the NBRF-PIR data bank. However, the higher homologies (up to about 28%) were obtained with enzymes that also utilize thiamine pyrophosphate as a cofactor.
摘要
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