Ikai I, Chance B, Kumar C
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia.
Free Radic Biol Med. 1994 May;16(5):539-45. doi: 10.1016/0891-5849(94)90053-1.
The effect of reactive oxygen generation on intact livers was studied. Production of reactive oxygen species in perfused livers isolated from normal and endotoxin-treated rats was measured using chemically enhanced chemiluminescence. The resting state chemiluminescence of the livers increased on endotoxin administration and was maximal about 6 h after treatment. Chemiluminescence from the livers was further stimulated severalfold by inclusion of phorbol myristate acetate in the perfusion medium, reaching maximum intensity 3 h after endotoxin treatment. Oxygen consumption by the endotoxin-treated liver showed a transient increase followed by a significant decrease on phorbol myristate acetate stimulation, which was inhibited by dexamethasone. These results are consistent with the occurrence of a respiratory burst followed by oxygen-radical-species-induced vasoconstriction in the intact perfused liver. The evaluation of reactive oxygen species by resident and accumulated macrophages in the intact liver is made possible by these studies, and related effects on the liver could be conveniently and quantitatively followed using this model.
研究了活性氧生成对完整肝脏的影响。使用化学增强化学发光法测量从正常大鼠和内毒素处理大鼠分离的灌注肝脏中活性氧的产生。内毒素给药后，肝脏的静息态化学发光增加，治疗后约6小时达到最大值。通过在灌注培养基中加入佛波酯肉豆蔻酸酯，肝脏的化学发光进一步被刺激数倍，在内毒素治疗后3小时达到最大强度。内毒素处理的肝脏的耗氧量在佛波酯肉豆蔻酸酯刺激后出现短暂增加，随后显著下降，地塞米松可抑制这种下降。这些结果与完整灌注肝脏中发生呼吸爆发继而氧自由基诱导的血管收缩一致。这些研究使得评估完整肝脏中驻留和聚集的巨噬细胞产生的活性氧成为可能，并且使用该模型可以方便且定量地追踪对肝脏的相关影响。
