Kasravi F B, Gebreselassie D, Adawi D, Wang L, Molin G, Bengmark S, Jeppsson B
Department of Surgery, Lund University, Sweden.
J Surg Res. 1996 Apr;62(1):63-8. doi: 10.1006/jsre.1996.0174.
The effect of endotoxin and Lactobacillus R2LC pretreatment in acute liver injury, induced by D-galactosamine was studied. Administration of D-galactosamine resulted in much higher luminescent activity than the control (49.24 mV vs 19.75 mV), while there was no change detected on the metabolic response to phorbol-12-myristate-13-acetate (PMA) stimulation in the calorimeter. Pretreatment of animals with intraperitoneal administration of heat-killed Lactobacillus resulted in higher oxygen free radical production (105.46 mV) and a much higher metabolic response to PMA in microcalorimeter (24.63 pW per cell vs 13.03 in the control). On the other hand pretreatment with endotoxin resulted in higher luminometric response to PMA compared to liver injury group (65.87 mV), and no response in the microcalorimeter at all. Phagocytic activity was decreased from 86 to 59% after administration of D-galactosamine, and was not significantly influenced by any of the pretreatment modalities. This experiment showed that pretreatment with Lactobacillus resulted in increased macrophage metabolic activity, while endotoxin pretreatment rendered the macrophages unresponsive to subsequent stimulation. These findings may explain why pretreatment with endotoxin is beneficial in acute liver injury induced by D-galactosamine, but not with gram-positive bacteria including Lactobacillus.
研究了内毒素和R2LC乳酸杆菌预处理对D-半乳糖胺诱导的急性肝损伤的影响。给予D-半乳糖胺后，其发光活性比对照组高得多（49.24 mV对19.75 mV），而在量热计中对佛波酯-12-肉豆蔻酸酯-13-乙酸酯（PMA）刺激的代谢反应未检测到变化。对动物进行腹腔注射热灭活乳酸杆菌预处理后，产生了更高的氧自由基产量（105.46 mV），并且在微量量热计中对PMA的代谢反应也高得多（每个细胞24.63 pW对对照组的13.03）。另一方面，与肝损伤组相比，内毒素预处理导致对PMA的发光响应更高（65.87 mV），而在微量量热计中完全没有反应。给予D-半乳糖胺后吞噬活性从86%降至59%，且不受任何预处理方式的显著影响。该实验表明，乳酸杆菌预处理导致巨噬细胞代谢活性增加，而内毒素预处理使巨噬细胞对后续刺激无反应。这些发现可能解释了为什么内毒素预处理对D-半乳糖胺诱导的急性肝损伤有益，但对包括乳酸杆菌在内的革兰氏阳性菌预处理则不然。
