Strauss G, Vignali D A, Schönrich G, Hämmerling G J
Tumor Immunology Program, German Cancer Research Center, Heidelberg.
Immunogenetics. 1994;40(2):104-8.
The establishment of HLA transgenic mice as models for autoimmune disorders requires that the HLA molecules can be efficiently recognized and mediate positive and negative selection of mouse T cells. This question was investigated in DR3(DRw17) transgenic mice back-crossed to the B10.Q(H-2q) strain which does not form mixed mouse-human class II heterodimers. Here we report that efficient negative selection on DR3(DRw17) molecules was observed for v beta 5, 11, and 13 subpopulations of CD4+T cells, but not for v beta 4, 7, 8, 9, and 10. v beta 5 and 11 cells are also negatively selected by mouse class II E molecules which is the structural homologue to DR molecules. Positive selection on DR3(DRw17) was only observed for v beta 6 cells but this was less efficient than positive selection of v beta 6 cells by E molecules. The data indicate that DR3(DRw17) molecules select similar subgroups of mouse T cells as E molecules although with slightly different efficiency.
将 HLA 转基因小鼠作为自身免疫性疾病模型来建立，要求 HLA 分子能够被有效识别并介导小鼠 T 细胞的阳性和阴性选择。在与不形成混合的小鼠 - 人类 II 类异二聚体的 B10.Q(H - 2q)品系回交的 DR3(DRw17)转基因小鼠中研究了这个问题。在此我们报告，对于 CD4 + T 细胞的 vβ5、11 和 13 亚群，观察到了对 DR3(DRw17)分子的有效阴性选择，但对于 vβ4、7、8、9 和 10 则没有。vβ5 和 11 细胞也被小鼠 II 类 E 分子阴性选择，E 分子是 DR 分子的结构同源物。仅在 vβ6 细胞中观察到了对 DR3(DRw17)的阳性选择，但这比 E 分子对 vβ6 细胞的阳性选择效率低。数据表明，DR3(DRw17)分子选择的小鼠 T 细胞亚群与 E 分子相似，尽管效率略有不同。
