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血清中丁型肝炎病毒RNA的化学发光杂交检测法。

Chemiluminescent hybridization assay for hepatitis delta virus RNA in serum.

作者信息

Gallagher M, Morris T T, Fields H A

机构信息

Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.

出版信息

J Clin Microbiol. 1994 Apr;32(4):1112-4. doi: 10.1128/jcm.32.4.1112-1114.1994.

DOI:10.1128/jcm.32.4.1112-1114.1994
PMID:8027326
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267200/
Abstract

A nonradioactive hybridization assay for the detection of hepatitis delta virus RNA in serum is described. This assay utilizes a digoxigenin-labeled RNA hybridization probe and chemiluminescent immunodetection. The probe can detect as little as 0.4 pg of cDNA with a 60-min exposure. Results obtained are in agreement with serological indicators of hepatitis delta virus infection and are comparable to those obtained by hybridization assays employing radioactively labeled RNA.

摘要

本文描述了一种用于检测血清中丁型肝炎病毒RNA的非放射性杂交检测方法。该检测方法使用地高辛标记的RNA杂交探针和化学发光免疫检测技术。该探针在60分钟曝光时间下能检测低至0.4 pg的cDNA。获得的结果与丁型肝炎病毒感染的血清学指标一致，并且与使用放射性标记RNA的杂交检测方法所获得的结果相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87c7/267200/8265067cea72/jcm00004-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87c7/267200/5aa7ede5fe84/jcm00004-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87c7/267200/8265067cea72/jcm00004-0269-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87c7/267200/5aa7ede5fe84/jcm00004-0269-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87c7/267200/8265067cea72/jcm00004-0269-b.jpg

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本文引用的文献

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Delta virus infection and severe hepatitis. An epidemic in the Yucpa Indians of Venezuela.德尔塔病毒感染与重症肝炎。委内瑞拉尤卡帕印第安人的一场流行病。
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Comparison of radio-labeled DNA probe with a nonisotopic probe for assay of serum hepatitis B virus DNA.
放射性标记DNA探针与非同位素探针检测血清乙肝病毒DNA的比较。
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Type D hepatitis: the clinical significance of hepatitis D virus RNA in serum as detected by a hybridization-based assay.丁型肝炎：基于杂交检测法检测血清中丁型肝炎病毒RNA的临床意义
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A nonradioactive riboprobe assay for the detection of hepatitis B virus DNA in human sera.一种用于检测人血清中乙型肝炎病毒DNA的非放射性核糖核酸探针检测法。
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