de Lamballerie X, Chapel F, Vignoli C, Zandotti C
Laboratoire de Virologie, Hôpital de la Timone, Marseille, France.
J Clin Pathol. 1994 May;47(5):466-7. doi: 10.1136/jcp.47.5.466.
With both a classic DNA preparation protocol (including removal of paraffin wax and protein digestion) and a DNA extraction protocol with Chelex 100, the hepatitis B virus genome was searched for using the polymerase chain reaction (PCR) in 30 samples of paraffin wax embedded liver tissue from patients with chronic hepatitis. The classic protocol was more sensitive than the rapid Chelex 100 procedure (10 v six positive samples). A third protocol, including removal of paraffin wax, protein digestion, and Chelex 100 treatment of the digestion solution before PCR, was more sensitive than the others (16 positive samples). It is concluded that it could therefore be helpful for PCR analysis of paraffin wax embedded liver tissue.
采用经典的DNA制备方案（包括去除石蜡和蛋白质消化）以及使用Chelex 100的DNA提取方案，通过聚合酶链反应（PCR）在30例慢性肝炎患者石蜡包埋肝组织样本中检测乙型肝炎病毒基因组。经典方案比快速Chelex 100方法更敏感（分别为10例和6例阳性样本）。第三种方案，包括去除石蜡、蛋白质消化以及在PCR前对消化液进行Chelex 100处理，比其他方案更敏感（16例阳性样本）。由此得出结论，这对于石蜡包埋肝组织的PCR分析可能是有帮助的。
