一种使用“Chelex 100”的适用于基因扩增的一步法微生物DNA提取方法。
A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification.
作者信息
de Lamballerie X, Zandotti C, Vignoli C, Bollet C, de Micco P
机构信息
Laboratoire de Microbiologie et d'Hygiène Hospitalières, Hôpital Salvator, Marseille, France.
出版信息
Res Microbiol. 1992 Oct;143(8):785-90. doi: 10.1016/0923-2508(92)90107-y.
PMID:1298031
Abstract
"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.
摘要
“Chelex 100”螯合树脂先前已被提议用于快速提取人类DNA以进行聚合酶链反应。文中给出了从培养物或临床样本中快速提取细菌和病毒DNA的方案。所获得的DNA样本适用于聚合酶链反应。
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