Hamernik K L
US Environmental Protection Agency, Office of Pesticide Programs, Washington, DC 20460.
J Appl Toxicol. 1994 Mar-Apr;14(2):131-4. doi: 10.1002/jat.2550140215.
The US Environmental Protection Agency now requires ocular toxicity testing to support the registration of organophosphorus pesticides. As a first step toward guideline development for the conduct of these studies, preliminary protocols for ocular toxicity testing in the non-rodent and rodent are being proposed by the Office of Pesticide Programs. Proposed protocol parameters include determination of animal health status, measurement of plasma, erythrocyte and retinal cholinesterase activities, ocular assessment by routine ophthalmological examination, slit lamp biomicroscopy, fundic observations, tonometry, electroretinography and determination of objective refractivity, pupillary response and tracking. Gross and detailed histopathological examinations of ocular system components would also be conducted. Associated questions and concerns with regard to ocular toxicity testing are presented. The Agency plans to hold a workshop in the near future to discuss issues related to protocol refinement and guideline development.
美国环境保护局现要求进行眼毒性测试，以支持有机磷农药的注册。作为制定这些研究指南的第一步，农药项目办公室正在提议非啮齿动物和啮齿动物眼毒性测试的初步方案。提议的方案参数包括确定动物健康状况、测量血浆、红细胞和视网膜胆碱酯酶活性、通过常规眼科检查、裂隙灯生物显微镜检查、眼底观察、眼压测量、视网膜电图以及客观屈光测定、瞳孔反应和追踪进行眼部评估。还将对眼部系统组件进行大体和详细的组织病理学检查。文中还提出了与眼毒性测试相关的问题和担忧。该机构计划在不久的将来举办一次研讨会，以讨论与方案完善和指南制定相关的问题。
