Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.
作者信息
Sanz-Aparicio J, Romero A, Martinez-Ripoll M, Madarro A, Flors A, Polaina J
机构信息
Departamento de Cristalografía, Instituto de Química-Física Rocasolano C.S.I.C., Madrid, Spain.
出版信息
J Mol Biol. 1994 Jul 15;240(3):267-70. doi: 10.1006/jmbi.1994.1441.
PMID:8028010
Abstract
The enzyme encoded by the bgIA gene of Bacillus polymyxa, a type I beta-glucosidase belonging to family I of glycosyl hydrolases, has been purified to homogeneity from an Escherichia coli culture which overexpressed the gene, and crystallized. The crystals, which diffract to 3.0 A resolution, belong to the orthorhombic space group C222(1). The cell dimensions are a = 155.4 A, b = 209.4 A, c = 209.7 A.
摘要
Zotero 插件