来自链霉菌属（ATCC 11238）的重组β-葡萄糖苷酶二级结构的预测及傅里叶变换红外光谱估计
Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant beta-glucosidase from Streptomyces sp. (ATCC 11238).
作者信息
Perez-Pons J A, Padros E, Querol E
机构信息
Institut de Biologica Fonamental, Universitat Autònoma de Barcelona, Spain.
出版信息
Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):791-4. doi: 10.1042/bj3080791.
Abstract
The secondary structure of a recombinant beta-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% alpha-helix, 30% beta-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% alpha-helix, 23% beta-sheet, 31% reverse turns and 11% non-ordered structures).
摘要
来自链霉菌属（ATCC 11238）的重组β-葡萄糖苷酶（EC 3.2.1.21）的二级结构已通过计算机算法进行预测，并通过傅里叶变换红外光谱进行估算。从解卷积红外光谱的曲线拟合来看，二级结构类别的最可能分布似乎约为34%的α-螺旋、30%的β-折叠、25%的反向转角和11%的非有序结构。这些数据与计算机预测的数据（35%的α-螺旋、23%的β-折叠、31%的反向转角和11%的非有序结构）吻合良好。
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