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Determination of protein secondary structure by Fourier transform infrared spectroscopy: a critical assessment.利用傅里叶变换红外光谱法测定蛋白质二级结构：一项批判性评估。
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Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy.通过傅里叶变换红外光谱法对溶液中蛋白质结构进行的定量研究。
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Crystallization and preliminary crystallographic analysis of the cyanogenic beta-glucosidase from the white clover Trifolium repens L.白三叶草（Trifolium repens L.）中含氰β-葡萄糖苷酶的结晶及初步晶体学分析
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Identification of active site carboxylic residues in Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase by site-directed mutagenesis.通过定点诱变鉴定地衣芽孢杆菌1,3-1,4-β-D-葡聚糖4-葡聚糖水解酶中的活性位点羧基残基。
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Functional and structural role of a tryptophan generally observed in protein-carbohydrate interaction. TRP-62 of hen egg white lysozyme.色氨酸在蛋白质-碳水化合物相互作用中普遍观察到的功能和结构作用。鸡蛋清溶菌酶的TRP-62。
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来自链霉菌属（ATCC 11238）的重组β-葡萄糖苷酶二级结构的预测及傅里叶变换红外光谱估计

Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant beta-glucosidase from Streptomyces sp. (ATCC 11238).

作者信息

Perez-Pons J A, Padros E, Querol E

机构信息

Institut de Biologica Fonamental, Universitat Autònoma de Barcelona, Spain.

出版信息

Biochem J. 1995 Jun 15;308 ( Pt 3)(Pt 3):791-4. doi: 10.1042/bj3080791.

DOI:10.1042/bj3080791
PMID:8948434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1136794/
Abstract

The secondary structure of a recombinant beta-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% alpha-helix, 30% beta-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% alpha-helix, 23% beta-sheet, 31% reverse turns and 11% non-ordered structures).

摘要

来自链霉菌属（ATCC 11238）的重组β-葡萄糖苷酶（EC 3.2.1.21）的二级结构已通过计算机算法进行预测，并通过傅里叶变换红外光谱进行估算。从解卷积红外光谱的曲线拟合来看，二级结构类别的最可能分布似乎约为34%的α-螺旋、30%的β-折叠、25%的反向转角和11%的非有序结构。这些数据与计算机预测的数据（35%的α-螺旋、23%的β-折叠、31%的反向转角和11%的非有序结构）吻合良好。