Buchmiller T L, Shaw K S, Lam M L, Stokes R, Diamond J S, Fonkalsrud E W
Department of Surgery, UCLA School of Medicine 90024.
J Surg Res. 1994 Aug;57(2):274-9. doi: 10.1006/jsre.1994.1144.
To examine the effect of prenatal steroids on fetal intestinal maturation, eight pregnant rabbits received either dexamethasone (Dex) or saline (Cont) on Days 25-27 of a 31-day gestation. As the rabbit provides a model of growth retardation based on uterine position, fetuses were identified as favored (Fav) or runt (Runt), generating four study groups: ContFav, ContRunt, DexFav, and DexRunt. On Day 31 the small intestinal uptake of glucose and proline was measured by an everted sleeve technique. Additionally, lactase and maltase activity was determined. Small intestinal length and nutrient uptake was significantly increased in the Dex fetuses. Control runts had a trend to decreased levels of nutrient uptake when compared to their favored counterparts. This trend reversed in the Dex fetuses with runt nutrient uptake surpassing that of the favored fetus. A trend to increased enzyme activity of both lactase and maltase was demonstrated. This report provides the first description of maternal steroid administration causing a marked increase in fetal small intestinal length and glucose and proline absorption in an in vivo model of intrauterine growth retardation.
为研究产前使用类固醇对胎儿肠道成熟的影响，8只怀孕母兔在31天妊娠期的第25至27天接受了地塞米松（Dex）或生理盐水（对照）注射。由于兔子可作为基于子宫位置的生长受限模型，胎儿被分为优势胎儿（Fav）或发育迟缓胎儿（Runt），由此产生了四个研究组：对照优势胎儿组（ContFav）、对照发育迟缓胎儿组（ContRunt）、地塞米松优势胎儿组（DexFav）和地塞米松发育迟缓胎儿组（DexRunt）。在第31天，采用外翻肠囊技术测量小肠对葡萄糖和脯氨酸的摄取。此外，还测定了乳糖酶和麦芽糖酶的活性。地塞米松处理的胎儿小肠长度和营养物质摄取显著增加。与对照优势胎儿相比，对照发育迟缓胎儿的营养物质摄取水平有降低趋势。在地塞米松处理的胎儿中，这种趋势发生了逆转，发育迟缓胎儿的营养物质摄取超过了优势胎儿。同时发现乳糖酶和麦芽糖酶的活性有增加趋势。本报告首次描述了在子宫内生长受限的体内模型中，母体给予类固醇会导致胎儿小肠长度以及葡萄糖和脯氨酸吸收显著增加。
