Gao G, Johansson U, Rundquist I, Ollinger K
Department of Pathology, Faculty of Health Sciences, University of Linköping, Sweden.
Mech Ageing Dev. 1994 Jan;73(1):79-86. doi: 10.1016/0047-6374(94)90040-x.
Lipofuscin-specific autofluorescence of living, cultured rat neonatal myocardial myocytes was studied with respect to intensity and spectral characteristics. The autofluorescence emission spectrum changed over time indicating continuously ongoing intramolecular reorganisation of the pigment. Moreover, as compared with formaldehyde-fixed material native lipofuscin produced a considerable red-shifted autofluorescence, indicating formaldehyde-induced molecular modification or quenching of certain parts of the spectrum. The relationship between oxidative stress and lipofuscinogenesis was confirmed because the rate of lipofuscin accumulation was increased when cells were grown at increasing ambient oxygen concentrations.
对培养的新生大鼠心肌细胞活体中的脂褐素特异性自发荧光的强度和光谱特征进行了研究。自发荧光发射光谱随时间变化，表明色素分子内持续进行着重组。此外，与甲醛固定的材料相比，天然脂褐素产生了明显的红移自发荧光，表明甲醛诱导了光谱某些部分的分子修饰或猝灭。氧化应激与脂褐素生成之间的关系得到了证实，因为当细胞在不断升高的环境氧浓度下生长时，脂褐素积累速率增加。
