Terman Alexei, Brunk Ulf T
Division of Pathology II, Faculty of Health Sciences, Linköping University, SE-581 85 Linköping, Sweden.
Phytother Res. 2002 Mar;16(2):180-2. doi: 10.1002/ptr.822.
Polbax, a water-soluble extract of fresh pollen grains and pistils, was tested for its ability to influence the accumulation of lipofuscin (age pigment) in cultured neonatal rat cardiac myocytes. Exposure for 3 weeks to Polbax at concentrations of 0.1, 1.0 or 10 mg/L decreased lipofuscin accumulation morphometrically assayed using laser scanning microscopy images (green excitation light) of formaldehyde-fixed cells, by 24%, 41% or 43%, respectively. Based on the knowledge that oxidative stress and iron-catalysed peroxidation play an important role in lipofuscinogenesis, we suggest that Polbax may slow lipofuscin formation due to antioxidant activities, perhaps involving intralysosomal dismutation of superoxide produced by autophagocytosed mitochondria and/or iron-chelation.
Polbax是新鲜花粉粒和雌蕊的水溶性提取物，对其影响培养的新生大鼠心肌细胞中脂褐素（老年色素）积累的能力进行了测试。在浓度为0.1、1.0或10mg/L的条件下，将细胞暴露于Polbax 3周，通过对甲醛固定细胞的激光扫描显微镜图像（绿色激发光）进行形态计量分析，结果显示脂褐素积累分别减少了24%、41%或43%。基于氧化应激和铁催化的过氧化在脂褐素形成中起重要作用这一认识，我们认为Polbax可能由于其抗氧化活性而减缓脂褐素的形成，这可能涉及自噬吞噬的线粒体产生的超氧化物在溶酶体内的歧化反应和/或铁螯合作用。
