Sugihara A, Shimada Y, Nakamura M, Nagao T, Tominaga Y
Osaka Municipal Technical Research Institute, Japan.
Protein Eng. 1994 Apr;7(4):585-8. doi: 10.1093/protein/7.4.585.
Geotrichum candidum ATCC34614 produces a major (I) and three minor (II, III and IV) forms of lipase with similar molecular masses, but different positional and fatty acid specificities. The major and one of the minor (II) forms were confirmed to cleave both the inside and outside ester bonds of triolein indiscriminately. The non-positional specificity was also retained on 1,3-dipalmitoyl-2-oleoyl-glycerol (POP) hydrolysis. In contrast, the remaining two forms (III and IV) showed unusual positional specificity; they cleaved the inside (2-position) ester bond of triolein at nearly twice the rate of cleavage at the 1(3)-position. The preference for the inside ester bond was increased upon POP hydrolysis.
白地霉ATCC34614产生一种主要的脂肪酶（I）和三种次要的脂肪酶（II、III和IV），它们分子量相似，但位置特异性和脂肪酸特异性不同。主要的脂肪酶形式和一种次要的脂肪酶形式（II）被证实可无差别地切割三油酸甘油酯的内部和外部酯键。在水解1,3 - 二棕榈酰 - 2 - 油酰 - 甘油（POP）时也保留了非位置特异性。相比之下，其余两种形式（III和IV）表现出不同寻常的位置特异性；它们切割三油酸甘油酯内部（2 - 位）酯键的速率几乎是切割1（3） - 位酯键速率的两倍。在水解POP时，对内部酯键的偏好性增加。
