Kapur R, Evans D L, Harris D T
Department of Microbiology and Immunology, University of Arizona, Tucson 85721.
Scand J Immunol. 1994 Jul;40(1):50-6. doi: 10.1111/j.1365-3083.1994.tb03432.x.
Using a novel anti-natural killer (NK) cell monoclonal antibody (MoAb), we have recently identified an evolutionary conserved function-associated molecule (FAM) present on fish, rat and human NK cells. This molecule is involved in NK cell function as anti-FAM MoAbs inhibit cytotoxicity, stimulate lymphokine secretion and inhibit conjugate formation between effector cells and target cells. We now have examined murine NK cells for the presence of this structure. It was observed by two-colour flow cytometric analysis that the anti-FAM MoAb 5C6 specifically bound to a subpopulation of nylon wool non-adherent splenic lymphocytes (19-20%). The expression of the FAM molecule was restricted to NK cells that expressed the NK1.1 antigen. Neither T cells, B cells, nor macrophages reacted with the anti-FAM MoAb. Analysis of FAM expression in various lymphoid tissues revealed that splenocytes expressed the greatest numbers of MoAb(+) cells. Generation of lymphokine-activated killer (LAK) cells and adherent lymphokine-activated killer (ALAK) cells resulted in higher levels of FAM expression. The anti-FAM MoAb 5C6 also detected the presence of FAM on fresh SCID NK cells. It was demonstrated that the anti-FAM MoAb 5C6 inhibited the lysis of target cells by endogenous NK cells, activated NK cells, 5d LAK cells, ALAK cells and SCID NK cells. Moreover, conjugate assays demonstrated involvement of this molecule in recognition between NK cells and target cells.
利用一种新型抗自然杀伤（NK）细胞单克隆抗体（MoAb），我们最近在鱼类、大鼠和人类NK细胞上鉴定出一种进化保守的功能相关分子（FAM）。该分子参与NK细胞功能，因为抗FAM MoAb可抑制细胞毒性、刺激淋巴因子分泌并抑制效应细胞与靶细胞之间的结合形成。我们现在已检测了小鼠NK细胞中这种结构的存在情况。通过双色流式细胞术分析观察到，抗FAM MoAb 5C6特异性结合于尼龙毛非黏附性脾淋巴细胞亚群（19 - 20%）。FAM分子的表达仅限于表达NK1.1抗原的NK细胞。T细胞、B细胞和巨噬细胞均不与抗FAM MoAb发生反应。对各种淋巴组织中FAM表达的分析显示，脾细胞表达的MoAb(+)细胞数量最多。淋巴因子激活的杀伤（LAK）细胞和黏附性淋巴因子激活的杀伤（ALAK）细胞的产生导致FAM表达水平升高。抗FAM MoAb 5C6也检测到新鲜SCID NK细胞上存在FAM。已证明抗FAM MoAb 5C6可抑制内源性NK细胞、活化NK细胞、5d LAK细胞、ALAK细胞和SCID NK细胞对靶细胞的裂解。此外，结合试验证明该分子参与NK细胞与靶细胞之间的识别。
