Harris D T, Jaso-Friedmann L, Devlin R B, Koren H S, Evans D L
Department of Microbiology & Immunology, University of Arizona, Tucson 85721.
Scand J Immunol. 1992 Mar;35(3):299-309. doi: 10.1111/j.1365-3083.1992.tb02862.x.
Experiments were performed to analyse the natural killer cell (NK)-like cytotoxicity frequently expressed by human antigen-specific cytolytic T lymphocytes (CTL). To this end, several monoclonal antibodies (MoAbs) previously shown to identify a novel function-associated molecule (FAM) involved in human NK function were utilized. Flow cytometry revealed that these MoAbs reacted with the majority of human NK, but only with a subpopulation of CTL isolated from primary mixed lymphocyte cultures. Preincubation of CTL with the MoAbs inhibited the NK-like lysis of K562 targets. Experiments with anti-CD3 MoAb demonstrated that neither the NK-like cytotoxicity of CTL nor the lytic activity of NK were mediated by the CD3 complex. Expression of the novel FAM was found to develop in T-cell cultures at the time that NK-like cytotoxicity was observed. Repeated in vitro antigenic stimulation of CTL was shown to result in loss of NK-like cytotoxicity, as well as loss of the FAM on the CTL surface. Thus, NK-like cytotoxicity displayed by antigen-specific CTL appears to be mediated by a novel FAM that is identical to that structure found on NK.
开展实验以分析人类抗原特异性细胞毒性T淋巴细胞（CTL）频繁表达的自然杀伤细胞（NK）样细胞毒性。为此，利用了几种先前已证明可识别参与人类NK功能的新型功能相关分子（FAM）的单克隆抗体（MoAb）。流式细胞术显示，这些MoAb与大多数人类NK反应，但仅与从原发性混合淋巴细胞培养物中分离出的CTL亚群反应。用MoAb对CTL进行预孵育可抑制K562靶标的NK样裂解。抗CD3 MoAb实验表明，CTL的NK样细胞毒性和NK的裂解活性均不由CD3复合物介导。发现新型FAM的表达在观察到NK样细胞毒性时在T细胞培养物中出现。反复体外抗原刺激CTL可导致NK样细胞毒性丧失，以及CTL表面FAM丧失。因此，抗原特异性CTL显示的NK样细胞毒性似乎由一种与NK上发现的结构相同的新型FAM介导。
