Duchemin J, Pittet J L, Tartary M, Béguin S, Gaussem P, Alhenc-Gelas M, Aiach M
INSERM CJF 91-01, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris V, France.
Thromb Haemost. 1994 Mar;71(3):331-8.
Activated protein C reduces thrombin generation by inactivating factors V and VIII in the presence of protein S. This prompted us to develop an assay which would allow specific exploration of this reaction. The total amount of thrombin formed in plasma after activation by tissue factor and phospholipids was reduced by adding thrombomodulin. This addition allowed protein C to be activated by endogenous thrombin. The inhibition of thrombin generation (ITG) due to protein C activation could be measured by comparing thrombin formation in the presence and in the absence of thrombomodulin. ITG increased with both protein C and protein S concentrations. Normal values of ITG expressed as a percentage were between 40 and 65% and were not influenced by age or sex. ITG increased in patients under heparin therapy, decreased in patients under oral anticoagulant therapy and was decreased in women using oral contraceptives. This method could be used for screening patients for protein C and protein S deficiencies.
活化蛋白C在蛋白S存在的情况下，通过使因子V和VIII失活来减少凝血酶的生成。这促使我们开发一种能够特异性探究该反应的检测方法。通过添加血栓调节蛋白，可减少组织因子和磷脂激活后血浆中形成的凝血酶总量。这种添加使得蛋白C能够被内源性凝血酶激活。通过比较存在和不存在血栓调节蛋白时的凝血酶形成情况，可测量由于蛋白C激活导致的凝血酶生成抑制（ITG）。ITG随蛋白C和蛋白S浓度的增加而增加。以百分比表示的ITG正常值在40%至65%之间，且不受年龄或性别的影响。肝素治疗的患者ITG增加，口服抗凝剂治疗的患者ITG降低，使用口服避孕药的女性ITG降低。该方法可用于筛查蛋白C和蛋白S缺乏的患者。
