Vaz Júnior I da S, Ozaki L S, Masuda A
Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, Brazil.
Vet Parasitol. 1994 Mar;52(1-2):71-8. doi: 10.1016/0304-4017(94)90037-x.
Five cattle were each experimentally infested with 30,000 Boophilus microplus larvae and their humoral immune response to salivary gland, gut, embryo and larval extracts during infestation were determined by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Serum antibodies to embryo extract were detected by ELISA 21 days after infestation and antibodies to the other extracts 28 days following infestation. Using Western blot analysis, new bands were recognized and others disappeared during infestation. One low molecular weight band (10,000) was detected in embryo extract 14 days after infestation and in other extracts 28 days after infestation; another band (12,000 mol. wt.) in embryo, larval and salivary gland extracts was detected at the same time as the 10,000 mol. wt. band. These results suggest that common antigens are present in different tissues of B. microplus.
五头牛每头都被实验性地接种了30,000只微小牛蜱幼虫，并通过酶联免疫吸附测定（ELISA）和蛋白质印迹分析来确定它们在感染期间对唾液腺、肠道、胚胎和幼虫提取物的体液免疫反应。感染后21天通过ELISA检测到针对胚胎提取物的血清抗体，感染后28天检测到针对其他提取物的抗体。使用蛋白质印迹分析，在感染期间识别出了新的条带，其他条带消失。感染后14天在胚胎提取物中检测到一条低分子量条带（10,000），感染后28天在其他提取物中检测到；在胚胎、幼虫和唾液腺提取物中，另一条带（分子量12,000）与分子量10,000的条带同时被检测到。这些结果表明，微小牛蜱的不同组织中存在共同抗原。
