Vaz Júnior I da S, Martinez R H, Oliveira A, Heck A, Logullo C, Gonzales J C, Dewes H, Masuda A
Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Vet Parasitol. 1996 Mar;62(1-2):155-60. doi: 10.1016/0304-4017(95)00851-9.
The aim of the present work was to quantify the passage of bovine immunoglobulins into the hemolymph of the tick Boophilus microplus during the feeding process and to determine their antibody activity. The knowledge is of paramount importance when vector control or blocking of disease transmission is attempted by vaccination of cattle. Approximately 2% of bovine immunoglobulin present in the serum as determined by competitive ELISA was demonstrated in hemolymph of B. microplus and antibody activity against an antigen of B. microplus in the hemolymph of ticks fed on bovine immunized with the antigen purified from tick eggs was detected by Western blot assay. The antibody reactivity detected against the B. microplus antigen showed that functional antibodies are present in the hemolymph of fully engorged ticks for at least 48 h after completing the parasitic life cycle.
本研究的目的是量化在取食过程中牛免疫球蛋白进入微小牛蜱血淋巴的情况，并确定其抗体活性。当试图通过给牛接种疫苗来控制病媒或阻断疾病传播时，这一知识至关重要。通过竞争性ELISA测定，微小牛蜱血淋巴中存在血清中约2%的牛免疫球蛋白，并且通过蛋白质印迹分析检测到，在以从蜱卵中纯化的抗原免疫的牛身上取食的蜱的血淋巴中，存在针对微小牛蜱抗原的抗体活性。检测到的针对微小牛蜱抗原的抗体反应性表明，饱血蜱在完成寄生生命周期后，其血淋巴中至少48小时内存在功能性抗体。
