Pöyry T, Hyypiä T, Horsnell C, Kinnunen L, Hovi T, Stanway G
Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
Virology. 1994 Aug 1;202(2):982-7. doi: 10.1006/viro.1994.1423.
Coxsackievirus A16 (CAV16) a member of the Enterovirus genus of Picornaviridae, is associated with hand-foot-and-mouth disease, a febrile papulovesicular rash of childhood. We have determined the complete nucleotide sequence of the genome of the prototype strain of CAV16 which consists of 7413 nucleotides plus the poly(A) tail. Alignment of the sequence with the previously studied enteroviruses showed that the genome organization is typical for a member of this virus genus. However, the predicted amino acid sequence of individual CAV16 proteins differed from those of all previously sequenced enteroviruses by 25-62%. The genomic sequence of CAV2 in the capsid and 2A polypeptide regions was also determined. It was found to differ from that of CAV16 by no more than 5-43%. The partial nucleotide sequence of enterovirus 71 in the VP1-2A region suggested that it is also closely related to CAV16. The results indicate that CAV16, CAV2, and enterovirus 71 represent a distinct genetic group of enteroviruses.
柯萨奇病毒A16（CAV16）是小RNA病毒科肠道病毒属的成员，与手足口病有关，手足口病是一种儿童发热性丘疹水疱疹。我们已经确定了CAV16原型株基因组的完整核苷酸序列，该序列由7413个核苷酸加上poly(A)尾组成。将该序列与先前研究的肠道病毒进行比对，结果表明其基因组结构是该病毒属成员的典型结构。然而，CAV16各蛋白的预测氨基酸序列与所有先前测序的肠道病毒的氨基酸序列相比，差异为25%至62%。还确定了CAV2在衣壳和2A多肽区域的基因组序列。发现它与CAV16的差异不超过5%至43%。肠道病毒71在VP1-2A区域的部分核苷酸序列表明，它也与CAV16密切相关。结果表明，CAV16、CAV2和肠道病毒71代表了一个独特的肠道病毒基因群。
