Barciszewska M Z
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Acta Biochim Pol. 1994;41(1):17-24.
In this paper transcriptional factor IIIA (TFIIIA) has been used as a probe for identity of three-dimensional-structure of eukaryotic 5S rRNAs. I was interested in finding a common motif in plant and Xenopus 5S rRNAs for TFIIIA recognition. I found that the two eukaryotic 5S rRNAs (from wheat germ and lupin seeds) are recognized by X. laevis TFIIIA and the data clearly suggest that these 5S rRNAs have very similar if not identical three-dimensional structures. Also effects of various conditions on stability of these complexes have been studied.
在本文中，转录因子IIIA（TFIIIA）已被用作探测真核生物5S rRNA三维结构一致性的探针。我感兴趣的是在植物和非洲爪蟾5S rRNA中找到一个共同的基序以供TFIIIA识别。我发现非洲爪蟾TFIIIA能够识别两种真核生物5S rRNA（来自小麦胚芽和羽扇豆种子），并且数据清楚地表明这些5S rRNA即便三维结构不完全相同，也非常相似。此外，还研究了各种条件对这些复合物稳定性的影响。
