[对其他抗病毒药物的敏感性及阿昔洛韦耐药的1型单纯疱疹病毒的胸苷激酶活性]

[Sensitivities to other antiviral drugs and thymidine kinase activity of aciclovir-resistant herpes simplex virus type 1].

作者信息

Nitta K, Shiota H, Naito T, Mimura Y

机构信息

Department of Ophthalmology, Tokushima University School of Medicine, Japan.

出版信息

Nippon Ganka Gakkai Zasshi. 1994 Jun;98(6):513-9.

PMID:8030564

Abstract

Sensitivity tests of various antiviral drugs on aciclovir-resistant strains of herpes simplex virus type 1 (HSV-1) were done in vitro. Activity of thymidine kinase (TK) of the aciclovir-resistant strains was also investigated. A strain of HSV-1 isolated from a patient with herpes labialis was grown in Eagle's minimum essential medium containing 10(-6)M aciclovir (ACV) and passaged 20 times. Then ACV-resistant strains of HSV-1 were obtained. Sensitivity tests of 5 anti-herpetic agents (ACV, ganciclovir, carbocyclic oxetanocin G, (S)-9- (3-hydroxy-2-phosphonylmethoxypropyl)adenine ((S)-HPMPA), and adenine arabinoside (ara-A)) were done on these ACV-resistant strains. The ACV-resistant strains were resistant to ganciclovir and carbocyclic oxetanocin G, but they were sensitive to (S)-HPMPA and ara-A. These ACV-resistant strains showed no detectable TK activity.

摘要

对1型单纯疱疹病毒（HSV-1）的阿昔洛韦耐药株进行了多种抗病毒药物的体外敏感性试验。同时还研究了阿昔洛韦耐药株的胸苷激酶（TK）活性。从一名唇疱疹患者分离出的一株HSV-1在含有10(-6)M阿昔洛韦（ACV）的伊格尔氏最低必需培养基中培养，并传代20次。然后获得了HSV-1的阿昔洛韦耐药株。对5种抗疱疹药物（阿昔洛韦、更昔洛韦、碳环氧杂环丁烷菌素G、(S)-9-(3-羟基-2-膦酰甲氧基丙基)腺嘌呤((S)-HPMPA)和阿糖腺苷（ara-A））对这些阿昔洛韦耐药株进行了敏感性试验。阿昔洛韦耐药株对更昔洛韦和碳环氧杂环丁烷菌素G耐药，但对(S)-HPMPA和ara-A敏感。这些阿昔洛韦耐药株未检测到TK活性。