Faas M M, Schuiling G A, Baller J F, Visscher C A, Bakker W W
Department of Pathology, University of Groningen, The Netherlands.
Am J Obstet Gynecol. 1994 Jul;171(1):158-64. doi: 10.1016/0002-9378(94)90463-4.
An animal model for preeclampsia was developed by means of an ultra-low-dose endotoxin infusion protocol in conscious pregnant rats.
Rats received a permanent jugular vein cannula on day 0 of pregnancy, through which endotoxin (1.0 micrograms/kg body weight) (n = 10) or saline solution (n = 6) was infused during 1 hour on day 14 of pregnancy. Blood pressure, albuminuria, and platelet counts were measured, and histopathologic studies was performed in these rats.
A significant increase of blood pressure (p < 0.05) and of urinary albumin excretion (p < 0.05) was observed in endotoxin-treated pregnant animals, in contrast to control pregnant rats receiving saline solution. Platelet coagulopathy was found and glomerular fibrinogen deposits could be detected only in the endotoxin-treated pregnant rats. In addition, the activity of the glomerular antithrombotic enzyme adenosine diphosphatase was decreased in endotoxin-treated pregnant rats compared with saline solution-treated pregnant rats.
Because histopathologic and clinical events in this model mimic predominant features of human preeclampsia, this model may enable further study into the pathophysiologic mechanisms of this complication of pregnancy.
通过对清醒妊娠大鼠采用超低剂量内毒素输注方案建立子痫前期动物模型。
大鼠在妊娠第0天接受永久性颈静脉插管，在妊娠第14天通过该插管在1小时内输注内毒素（1.0微克/千克体重）（n = 10）或生理盐水（n = 6）。测量血压、蛋白尿和血小板计数，并对这些大鼠进行组织病理学研究。
与接受生理盐水的对照妊娠大鼠相比，在内毒素处理的妊娠动物中观察到血压显著升高（p < 0.05）和尿白蛋白排泄显著增加（p < 0.05）。仅在内毒素处理的妊娠大鼠中发现血小板凝血病且可检测到肾小球纤维蛋白原沉积。此外，与生理盐水处理的妊娠大鼠相比，内毒素处理的妊娠大鼠肾小球抗血栓酶腺苷二磷酸酶的活性降低。
由于该模型中的组织病理学和临床事件模拟了人类子痫前期的主要特征，该模型可能有助于进一步研究妊娠该并发症的病理生理机制。
