感染后输卵管性不孕女性输卵管组织中沙眼衣原体的检测
Detection of Chlamydia trachomatis in fallopian tube tissue in women with postinfectious tubal infertility.
作者信息
Patton D L, Askienazy-Elbhar M, Henry-Suchet J, Campbell L A, Cappuccio A, Tannous W, Wang S P, Kuo C C
机构信息
Department of Obstetrics and Gynecology, University of Washington, Seattle 98195.
出版信息
Am J Obstet Gynecol. 1994 Jul;171(1):95-101. doi: 10.1016/s0002-9378(94)70084-2.
OBJECTIVE
Biopsy tissues from women with postinfectious tubal infertility were studied for the presence of Chlamydia trachomatis.
STUDY DESIGN
Tubal biopsy specimens from 25 women with postinfectious tubal infertility undergoing laparoscopy for repair of fallopian tubes were evaluated by culture, in situ hybridization. Immunocytochemistry, and transmission electron microscopy for the presence of Chlamydia trachomatis. Serum was also tested for Chlamydia trachomatis antibodies.
RESULTS
Chlamydia trachomatis was detected in postinfectious tubal biopsy specimens in three of 25 patients by culture, 12 of 24 by in situ hybridization, 15 of 22 by immunoperoxidase stain, and two of 10 by transmission electron microscopy. Serum antibody against Chlamydia trachomatis was detected in 15 of 21 patients.
CONCLUSION
Chlamydia trachomatis deoxyribonucleic acid or antigens were detected at a high percentage (19/24 women) in the biopsy tissues of the fimbrial and peritubal adhesions by in situ hybridization or immunoperoxidase stain, suggesting a persistent infection in these women even after antibiotic treatment.
目的
研究感染后输卵管性不孕女性活检组织中沙眼衣原体的存在情况。
研究设计
对25例因感染后输卵管性不孕接受腹腔镜输卵管修复术的女性的输卵管活检标本进行培养、原位杂交、免疫细胞化学及透射电子显微镜检查，以检测沙眼衣原体的存在。同时检测血清中的沙眼衣原体抗体。
结果
25例患者中，3例经培养在感染后输卵管活检标本中检测到沙眼衣原体；24例中12例经原位杂交检测到；22例中15例经免疫过氧化物酶染色检测到；10例中2例经透射电子显微镜检测到。21例患者中15例检测到抗沙眼衣原体血清抗体。
结论
通过原位杂交或免疫过氧化物酶染色，在伞端和输卵管周围粘连的活检组织中，沙眼衣原体脱氧核糖核酸或抗原的检出率较高（19/24例女性），提示这些女性即使在抗生素治疗后仍存在持续感染。
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