Cano R J, Borucki M K, Higby-Schweitzer M, Poinar H N, Poinar G O, Pollard K J
Biological Sciences Department, California Polytechnic State University, San Luis Obispo 93407.
Appl Environ Microbiol. 1994 Jun;60(6):2164-7. doi: 10.1128/aem.60.6.2164-2167.1994.
We report here the isolation of DNA from abdominal tissue of four extinct stingless bees (Proplebeia dominicana) in Dominican amber, PCR amplification of a 546-bp fragment of the 16S rRNA gene from Bacillus spp., and their corresponding nucleotide sequences. These sequences were used in basic local alignment search tool searches of nonredundant nucleic acid data bases, and the highest scores were obtained with 16S rRNA sequences from Bacillus spp. Phylogenetic inference analysis by the maximum-likelihood method revealed close phylogenetic relationships of the four presumed ancient Bacillus sequences with Bacillus pumilus, B. firmus, B. subtilis, and B. circulans. These four extant Bacillus spp. are commonly isolated from abdominal tissue of stingless bees. The close phylogenetic association of the extracted DNA sequences with these bee colonizers suggests that a similar bee-Bacillus association existed in the extinct species P. dominicana.
我们在此报告从多米尼加琥珀中四只已灭绝的无刺蜂（多米尼加原无刺蜂）的腹部组织中分离DNA，对芽孢杆菌属16S rRNA基因的一个546碱基对片段进行PCR扩增，以及它们相应的核苷酸序列。这些序列用于在非冗余核酸数据库中进行基本局部比对搜索工具搜索，并且与芽孢杆菌属的16S rRNA序列获得了最高分。通过最大似然法进行的系统发育推断分析表明，这四个推测的古代芽孢杆菌序列与短小芽孢杆菌、坚强芽孢杆菌、枯草芽孢杆菌和环状芽孢杆菌有密切的系统发育关系。这四种现存的芽孢杆菌属通常从无刺蜂的腹部组织中分离得到。提取的DNA序列与这些蜜蜂定殖菌的密切系统发育关联表明，在已灭绝的物种多米尼加原无刺蜂中存在类似的蜜蜂 - 芽孢杆菌关联。
