Carones F, Brancato R, Venturi E, Morico A
Department of Ophthalmology and Visual Sciences, San Raffaele Hospital, University of Milan, Italy.
Arch Ophthalmol. 1994 Jul;112(7):920-4. doi: 10.1001/archopht.1994.01090190068023.
To investigate whether 193-nm excimer laser photorefractive keratectomy for the correction of myopia can determine in vivo changes in the human corneal endothelium 3 and 12 months after surgery.
Contact wide-field specular biomicroscopy of the central corneal endothelium was performed on 76 eyes of 61 patients (aged 20 to 49 years; mean, 29.7 years) who underwent photorefractive keratectomy for the correction of myopia ranging between -1.75 and -13.50 diopters (mean, -6.60 D). The maximum estimated central depth of the photoablation was 113 microns. Photomicrographs of the endothelium taken before surgery were processed by means of computer-assisted morphometric analysis and compared with those obtained 3 and 12 months after surgery.
The mean (+/- SD) cell density was 2657 +/- 298 cells/mm2 preoperatively, 2672 +/- 286 cells/mm2 after 3 months (P = .670), and 2656 +/- 289 cells/mm2 after 1 year (P = .982). The mean (+/- SD) coefficient of cell area variation was 30.27% +/- 5.99% preoperatively, 28.25% +/- 5.21% after 3 months (P = .032), and 26.35% +/- 5.29% after 1 year (P = .001). The mean (+/- SD) percentage of hexagonal cells was 63.82% +/- 9.94% preoperatively, 64.02% +/- 10.53% after 3 months (P = .806), and 67.19% +/- 9.14% after 1 year (P = .025).
Photorefractive keratectomy caused no damage to the corneal endothelium. Postoperative improvements of the coefficient cell area variation and the percentage of hexagonal cell values may be related to corneal metabolic modifications or discontinuance of contact lens use after surgery.
研究193纳米准分子激光屈光性角膜切削术矫正近视后3个月和12个月时，能否确定人角膜内皮细胞的体内变化。
对61例（年龄20至49岁，平均29.7岁）接受屈光性角膜切削术矫正-1.75至-13.50屈光度（平均-6.60 D）近视的患者的76只眼进行中央角膜内皮细胞的接触式宽视野镜面显微镜检查。光凝消融的最大估计中央深度为113微米。术前拍摄的内皮细胞显微照片通过计算机辅助形态计量分析进行处理，并与术后3个月和12个月获得的照片进行比较。
平均（±标准差）细胞密度术前为2657±298个细胞/mm²，3个月后为2672±286个细胞/mm²（P = 0.670），1年后为2656±289个细胞/mm²（P = 0.982）。平均（±标准差）细胞面积变异系数术前为30.27%±5.99%，3个月后为28.25%±5.21%（P = 0.032），1年后为26.35%±5.29%（P = 0.001）。平均（±标准差）六边形细胞百分比术前为63.82%±9.94%，3个月后为64.02%±10.53%（P = 0.806），1年后为67.19%±9.14%（P = 0.025）。
屈光性角膜切削术未对角膜内皮细胞造成损害。术后细胞面积变异系数和六边形细胞值百分比的改善可能与角膜代谢改变或术后停止使用隐形眼镜有关。
