McPheron B A, Gasparich G E, Han H Y, Steck G J, Sheppard W S
Department of Entomology, Penn State University, University Park, Pennsylvania 16802-3508.
Biochem Genet. 1994 Feb;32(1-2):25-33. doi: 10.1007/BF00557236.
Molecular genetic research on the Mediterranean fruit fly, Ceratitis capitata, will provide tools to permit determination of source populations for new pest infestations. Restriction fragment length polymorphism (RFLP) of mitochondrial DNA provides some interpopulation discrimination. A restriction map, including the informative variable EcoRV and XbaI restriction sites, is constructed for the Mediterranean fruit fly, and several restriction sites are associated with specific gene regions based on polymerase chain reaction-RFLP and sequence analyses. A partial sequence of the mitochondrial 16S ribosomal RNA gene is reported.
对地中海实蝇（Ceratitis capitata）的分子遗传学研究将提供工具，以便确定新害虫侵扰的来源种群。线粒体DNA的限制性片段长度多态性（RFLP）提供了一些种群间的区分。构建了地中海实蝇的限制性图谱，包括信息丰富的可变EcoRV和XbaI限制性位点，并基于聚合酶链反应-RFLP和序列分析，确定了几个与特定基因区域相关的限制性位点。报道了线粒体16S核糖体RNA基因的部分序列。
