Chenault K D, Melcher U
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078-0454.
Biochimie. 1994;76(1):3-8. doi: 10.1016/0300-9084(94)90056-6.
A consensus nucleotide sequence of the DNA of nine isolates of cauliflower mosaic virus (CaMV) was used to examine variation of nucleotide sequence in CaMV. Variability in coding regions was lowest in open reading frames (ORFs) 1, 2, 3 and 5 and higher in ORFs 4 and 6. Silent substitutions were not uniformly distributed among the ORFs. The large intergenic region was also variable, particularly in loops and bulges of a predicted secondary structure for this region of the 35S RNA transcript. A profile of frequencies of the substitution of consensus nucleotides with other nucleotides revealed a deficit of A to G transitions and an excess of transversions involving A. Most insertions/deletions could be accounted for by template misalignment during replication. The results suggest that the major source of variation in CaMV DNA sequences is associated with replication by reverse transcription.
利用花椰菜花叶病毒（CaMV）九个分离株的DNA共有核苷酸序列来检测CaMV核苷酸序列的变异情况。编码区的变异性在开放阅读框（ORF）1、2、3和5中最低，而在ORF 4和6中较高。沉默替代在各个ORF中分布并不均匀。大的基因间隔区也存在变异，特别是在35S RNA转录本该区域预测二级结构的环和凸起处。共有核苷酸被其他核苷酸替代的频率图谱显示，A到G的转换不足，而涉及A的颠换过多。大多数插入/缺失可归因于复制过程中模板的错配。结果表明，CaMV DNA序列变异的主要来源与逆转录复制有关。
