Lichtinghagen R, Diedrich-Glaubitz R, von Hörsten B
Institut für Klinische Chemie I der Medizinischen Hochschule Hannover, Germany.
Eur J Clin Chem Clin Biochem. 1994 Mar;32(3):161-7. doi: 10.1515/cclm.1994.32.3.161.
A 183 base pairs or 153 base pairs DNA fragment from a repetitive region of the Bordetella pertussis genome was amplified in a polymerase chain reaction. The sensitivities of three different detection methods (Enzymun Test, silver stained polyacrylamide gel, ethidium bromide stained agarose gel) after amplification by polymerase chain reaction showed that both a one-time polymerase chain reaction (35 cycles) with Enzymun testing as well as a nested polymerase chain reaction with either of the electrophoresis methods have high levels of sensitivity for detection of the infectious organism in nasopharyngeal swabs. Smears from 53 children with whooping cough and from 50 children without infections were analysed, using these methods. 51 patients with whooping cough gave positive test results, while 2 of the sick patients and all the control children gave negative results.
在聚合酶链反应中扩增了来自百日咳博德特氏菌基因组重复区域的一段183个碱基对或153个碱基对的DNA片段。聚合酶链反应扩增后，三种不同检测方法（酶免疫试验、银染聚丙烯酰胺凝胶、溴化乙锭染色琼脂糖凝胶）的敏感性表明，单次聚合酶链反应（35个循环）结合酶免疫试验以及采用任何一种电泳方法的巢式聚合酶链反应，对检测鼻咽拭子中的感染性生物体均具有高灵敏度。使用这些方法对53例百日咳患儿和50例未感染儿童的涂片进行了分析。51例百日咳患者检测结果呈阳性，而2例患病患者和所有对照儿童检测结果呈阴性。
