Adler B K, Hajduk S L
Department of Medicine, University of Alabama at Birmingham, Alabama 35294.
Curr Opin Genet Dev. 1994 Apr;4(2):316-22. doi: 10.1016/s0959-437x(05)80060-7.
RNA editing is an essential post-transcriptional process that has been identified in an increasing number of eukaryotic organisms. In the past year, progress has been made in the development of in vitro systems to study the mechanism of RNA editing. Analysis of nucleotide insertion/deletion editing in trypanosome mitochondria has revealed the existence of putative editing intermediates in vivo and in vitro. The development of an in vitro editing system for mammalian apolipoprotein B mRNA has allowed the elucidation of both the sequence requirements and the biochemical mechanism of this form of RNA editing. In addition, recent work has underscored the diversity of RNAs whose structure and function are altered by post-translational editing reactions.
RNA编辑是一种重要的转录后过程，已在越来越多的真核生物中被发现。在过去的一年里，用于研究RNA编辑机制的体外系统的开发取得了进展。对锥虫线粒体中核苷酸插入/缺失编辑的分析揭示了体内和体外推定的编辑中间体的存在。哺乳动物载脂蛋白B mRNA体外编辑系统的开发使得阐明这种RNA编辑形式的序列要求和生化机制成为可能。此外，最近的研究强调了其结构和功能因翻译后编辑反应而改变的RNA的多样性。
