Mottolese M, Venturo I, Benevolo M, Di Filippo F, Lopez M, Bigotti A, Natali P G
Department of Pathology, Regina Elena Cancer Institute, Rome, Italy.
Melanoma Res. 1994 Feb;4(1):53-8. doi: 10.1097/00008390-199402000-00008.
The analysis of fine-needle aspirates and effusions has proved to be a valuable tool for the cytological identification of metastatic melanoma. Nevertheless, while malignant pigmented cells can be easily detected on cytological specimens, their identification may be very difficult in patients bearing amelanotic lesions. In the present study we have evaluated whether this limitation can be overcome using two monoclonal antibodies (mAbs) HMB45 and Ep1-3, which are highly specific for the melanocyte lineage. These reagents were assayed in immunocytochemical tests performed on cytological material obtained from 50 patients with a past history of melanoma and 463 bearing metastases from a cryptic primary tumour. These mAbs, employed in combination with a panel of monoclonal reagents with well-defined tumour specificity, may improve the accuracy of the conventional cytopathological diagnosis of melanoma metastases in the two groups of patients.
细针穿刺抽吸物和积液的分析已被证明是用于转移性黑色素瘤细胞学鉴定的一种有价值的工具。然而，虽然在细胞学标本上可以很容易地检测到恶性色素细胞，但在患有无色素性病变的患者中，其识别可能非常困难。在本研究中，我们评估了使用两种对黑素细胞谱系具有高度特异性的单克隆抗体（mAb）HMB45和Ep1-3是否可以克服这一局限性。在对从50例有黑色素瘤病史的患者以及463例患有隐匿性原发性肿瘤转移的患者获得的细胞学材料进行的免疫细胞化学试验中检测了这些试剂。这些mAb与一组具有明确肿瘤特异性的单克隆试剂联合使用，可能会提高两组患者中黑色素瘤转移的传统细胞病理学诊断的准确性。
