Rittweger R, Hermann K, Ring J
Department of Dermatology, University Hospital Eppendorf, University of Hamburg, Germany.
Int Arch Allergy Immunol. 1994 Jul;104(3):255-61. doi: 10.1159/000236674.
Immunoreactive angiotensin I (ANG I) and angiotensin II (ANG II) were measured in human urine, after purification on octadecasilyl-silica cartridges. The total daily excretion of ANG I and II in healthy volunteers was 292.2 +/- 62.5 and 12.2 +/- 2.5 pmol/24 h (mean +/- SEM; n = 14). No differences in the concentrations of ANG I or II were detected between females and males. Although lower levels of ANG I and II were found during the nighttime, no clear-cut circadian rhythm in the excretion of the peptides was found. ANG II was not degraded in acidified urine which shows the effective inhibition of ANG-II-degrading enzymes. Oral provocation tests (OPT) in patients with a history of anaphylactoid reactions (AR) to drugs, foods and food additives were associated with elevated ANG I and II concentrations when symptoms of anaphylaxis occurred. The excretion of ANG I increased by a factor of 7.8 +/- 2.4 and the excretion of ANG II by a factor of 6.1 +/- 1.6 (mean +/- SEM; n = 15). In patients with negative OPT and no clinical symptoms of anaphylaxis, the levels of ANG I and II remained unchanged (n = 26). It is concluded that angiotensin peptides play a role during the events of AR. The peptides may be considered as counteracting factors which stabilize cardiovascular functions.
在经十八烷基硅烷硅胶柱纯化后，测定人尿液中的免疫反应性血管紧张素I（ANG I）和血管紧张素II（ANG II）。健康志愿者中ANG I和ANG II的每日总排泄量分别为292.2±62.5和12.2±2.5 pmol/24小时（平均值±标准误；n = 14）。未检测到女性和男性之间ANG I或ANG II浓度的差异。尽管夜间发现ANG I和ANG II水平较低，但未发现这些肽排泄的明显昼夜节律。ANG II在酸化尿液中未降解，这表明ANG-II降解酶受到有效抑制。对药物、食物和食品添加剂有类过敏反应（AR）病史的患者进行口服激发试验（OPT）时，当出现过敏反应症状时，ANG I和ANG II浓度会升高。ANG I的排泄增加了7.8±2.4倍，ANG II的排泄增加了6.1±1.6倍（平均值±标准误；n = 15）。在OPT阴性且无过敏反应临床症状的患者中，ANG I和ANG II水平保持不变（n = 26）。结论是血管紧张素肽在AR事件中起作用。这些肽可被视为稳定心血管功能的对抗因子。
