Hermann K, Rittweger R, Ring J
Department of Dermatology, Klinikum Eppendorf, University of Hamburg, Germany.
Clin Exp Allergy. 1992 Sep;22(9):845-53. doi: 10.1111/j.1365-2222.1992.tb02830.x.
Human urine samples, purified on octadecasilyl-silica cartridges, contained immunoreactive angiotensin I, II, arginine vasopressin and oxytocin. The daily excretion of these peptides in healthy volunteers was 190.00 +/- 38.43 (n = 12), 17.48 +/- 3.09 (n = 12), 63.43 +/- 14.84 (n = 8) and 13.52 +/- 1.42 (n = 7) pmol/24 hr, respectively (mean +/- s.e.m.). Patients with a history of anaphylactoid reactions to drugs or food additives showed clinical symptoms such as urticaria, flush, nausea, dizziness and hypotension after oral provocation with cyanocobalamine, propyphenazone, acetylsalicylic acid and sodium benzoate. In five of the seven patients, angiotensin I and II were increased several fold in the urine fractions after symptoms were reported. The average increase in the urine concentration of both peptides was fourfold and 5.5-fold. In three out of five patients, the mean excretion of arginine vasopressin and oxytocin immunoreactive material was also elevated by a factor of 5.7 and 4.4, respectively. Oral provocation with a placebo failed to elicit anaphylactoid symptoms or an increase in the urine levels of angiotensin I or angiotensin II. Angiotensin I and angiotensin II-like immunoreactivity could be characterized on HPLC as Ile5-angiotensin I, Ile5-angiotensin II and angiotensin II metabolites. HPLC characterization of immunoreactive arginine vasopressin and oxytocin in two different gradient systems showed retention times different than the retention times of the corresponding synthetic standard peptides indicating that both peptides are not authentic AVP and OXT. These results suggest that angiotensin I and angiotensin II may be involved in the clinical events observed during some forms of anaphylactoid reactions.
经十八烷基硅烷键合硅胶柱纯化后的人尿样中含有免疫反应性血管紧张素I、II、精氨酸加压素和催产素。健康志愿者中这些肽的每日排泄量分别为190.00±38.43（n = 12）、17.48±3.09（n = 12）、63.43±14.84（n = 8）和13.52±1.42（n = 7）pmol/24小时（平均值±标准误）。有药物或食品添加剂类过敏样反应病史的患者，在口服钴胺素、保泰松、乙酰水杨酸和苯甲酸钠进行激发试验后，出现了荨麻疹、潮红、恶心、头晕和低血压等临床症状。在7例患者中的5例中，报告症状后尿样组分中的血管紧张素I和II增加了几倍。两种肽的尿浓度平均增加了4倍和5.5倍。在5例患者中的3例中，精氨酸加压素和催产素免疫反应性物质的平均排泄量也分别升高了5.7倍和4.4倍。口服安慰剂进行激发试验未引发过敏样症状或血管紧张素I或血管紧张素II尿水平升高。血管紧张素I和血管紧张素II样免疫反应性在高效液相色谱上可表征为异亮氨酸5-血管紧张素I、异亮氨酸5-血管紧张素II和血管紧张素II代谢产物。在两种不同梯度系统中对免疫反应性精氨酸加压素和催产素进行高效液相色谱表征，结果显示其保留时间与相应合成标准肽的保留时间不同，这表明这两种肽并非真正的精氨酸加压素和催产素。这些结果表明，血管紧张素I和血管紧张素II可能参与了某些形式的过敏样反应中观察到的临床事件。
