Segawa K, Ono K, Oka S, Jyo T, Kuroiwa A, Yamashita U
2nd Department of Internal Medicine, University of Occupational and Environmental Health School of Medicine, Kitakyusyu, Japan.
Int Arch Allergy Immunol. 1994 Jul;104(3):270-6. doi: 10.1159/000236676.
The activity of sea squirt antigen, one of the allergy-inducing substances for humans, on murine and human lymphocytes was studied in vitro. Sea squirt antigen stimulated normal mouse spleen cells to proliferate, as detected by [3H]-TdR incorporation, in a dose-dependent manner. The responder cells are B cells because the response was reduced by the treatment of spleen cells with anti-immunoglobulin antibody and complement and passing through a nylon wool column, but not with anti-Thy-1 antibody and complement. Spleen cells of C3H/HeJ mice, which are lipopolysaccharide low responders, were also stimulated as well as spleen cells of C3H/HeN mice, suggesting that this response is not due to lipopolysaccharide in the antigen fraction. Sea squirt antigen stimulated not only proliferative response of B cells, but also polyclonal immunoglobulin production. Furthermore, sea squirt antigen also stimulated human lymphocytes to proliferate and to produce immunoglobulin. All these results suggest that sea squirt antigen has mitogenic activity on B cells, and this ability is concerned with the induction of allergic reaction.
对人类过敏诱导物质之一的海鞘抗原在小鼠和人类淋巴细胞上的活性进行了体外研究。通过[³H]-TdR掺入检测发现，海鞘抗原能刺激正常小鼠脾细胞增殖，且呈剂量依赖性。应答细胞是B细胞，因为用抗免疫球蛋白抗体和补体处理脾细胞并通过尼龙毛柱后，反应减弱，而用抗Thy-1抗体和补体处理则无此现象。脂多糖低反应性的C3H/HeJ小鼠的脾细胞与C3H/HeN小鼠的脾细胞一样受到刺激，这表明该反应不是由于抗原组分中的脂多糖引起的。海鞘抗原不仅刺激B细胞的增殖反应，还刺激多克隆免疫球蛋白的产生。此外，海鞘抗原也刺激人类淋巴细胞增殖并产生免疫球蛋白。所有这些结果表明，海鞘抗原对B细胞具有促有丝分裂活性，且这种能力与过敏反应的诱导有关。
