Yoshida Y, Wang M Q, Liu J N, Shan B E, Yamashita U
Department of Immunology and Parasitology, Hiroshima University School of Medicine, Japan.
Int J Immunopharmacol. 1997 Jul;19(7):359-70. doi: 10.1016/s0192-0561(97)00076-3.
The effect of eight different Chinese medicinal herbs (CMHs) on lymphocytes was studied in vitro using murine spleen cells. Among the studied eight CMHs, Astragalus membranaceus and Oldenlandia diffusa markedly stimulated murine spleen cells to proliferate. The responder cells for CMHs were B cells, because the response was depleted by the treatment of spleen cells with anti-immunoglobulin (i.g.) antibody and complement and after purification by nylon wool column. This response was not due to contamination by lipopolysaccharide (LPS), because CMHs could stimulate C3H/HeJ spleen cells which are low responders to LPS. CMHs enhanced the production of Ig. CMHs also enhanced the induction of allo-antigen specific cytotoxic T lymphocytes. However, CMHs had no effect on natural killer cells. Furthermore, CMHs stimulated macrophages to produce interleukin-6 and tumor necrosis factor. The electroelution of the proteins from SDS-PAGE gel showed that the active components of Oldenlandia diffusa had an apparent molecular weight of 90-200 kD and were sensitive to pronase E and NaIO4 treatment, suggesting glycoproteins in nature. These results suggest that CMHs have immunomodulating activity in vitro and this activity could be used clinically for the modulation of immune responses.
利用小鼠脾细胞在体外研究了八种不同的中草药（CMHs）对淋巴细胞的作用。在所研究的八种CMHs中，黄芪和白花蛇舌草显著刺激小鼠脾细胞增殖。CMHs的反应细胞是B细胞，因为用抗免疫球蛋白（i.g.）抗体和补体处理脾细胞以及经尼龙毛柱纯化后反应会减弱。这种反应不是由于脂多糖（LPS）污染所致，因为CMHs能刺激对LPS反应较弱的C3H/HeJ脾细胞。CMHs增强了Ig的产生。CMHs还增强了同种异体抗原特异性细胞毒性T淋巴细胞的诱导。然而，CMHs对自然杀伤细胞没有影响。此外，CMHs刺激巨噬细胞产生白细胞介素-6和肿瘤坏死因子。从SDS-PAGE凝胶上进行蛋白质电洗脱显示，白花蛇舌草的活性成分表观分子量为90 - 200 kD，对链霉蛋白酶E和高碘酸钠处理敏感，提示其本质为糖蛋白。这些结果表明，CMHs在体外具有免疫调节活性，这种活性可在临床上用于调节免疫反应。
