绵羊IgE与抗人IgE的交叉反应性。

Cross-reactivity of ovine IgE with anti-human IgE.

作者信息

Yilmaz H, Morgan K L, Roe J M

机构信息

School of Veterinary Science, Department of Veterinary Medicine, Bristol, UK.

出版信息

Int Arch Allergy Immunol. 1994 Jul;104(3):277-80. doi: 10.1159/000236677.

DOI:10.1159/000236677

PMID:8032239

Abstract

Cross-reactivity between ovine and human IgE was investigated using monoclonal antibodies to human IgE heavy chain. Western immunoblotting, reverse cutaneous anaphylaxis (RCA), passive cutaneous anaphylaxis inhibition (PCAI) and reverse passive cutaneous anaphylaxis (RPCA) tests were all used to assess cross-reactivity between the monoclonals and ovine IgE. No cross-reactivity was demonstrated using Western immunoblotting, RCA and PCAI tests. However, evidence for homology in the region of the molecule concerned with mast cell binding was demonstrated by RPCA performed in sheep skin primed with human myeloma IgE.

摘要

利用抗人IgE重链单克隆抗体研究了绵羊和人IgE之间的交叉反应性。采用蛋白质免疫印迹法、反向皮肤过敏反应（RCA）、被动皮肤过敏反应抑制（PCAI）和反向被动皮肤过敏反应（RPCA）试验来评估单克隆抗体与绵羊IgE之间的交叉反应性。蛋白质免疫印迹法、RCA和PCAI试验均未显示交叉反应性。然而，在用人类骨髓瘤IgE致敏的绵羊皮肤中进行的RPCA试验证明了在与肥大细胞结合相关的分子区域存在同源性。