Moore F M, Simpson D
Department of Clinical Chemistry, Royal Infirmary, Edinburgh, Scotland, UK.
Br J Biomed Sci. 1993 Mar;50(1):57-9.
Following suggestions that spurious results occur with EMIT assays--so-called 'fliers'--many laboratories perform such assays in duplicate. In this study, using a Cobas-Bio centrifugal analyser and cost-effective assays developed in the authors' laboratory, complete EMIT assays were run, each with its own calibration curve, on three anti-epileptic drugs using plasma samples, and five drugs of abuse using urine samples. No serious spurious results were obtained. It is suggested that there is no inherent tendency for EMIT assays to produce 'fliers' and that when these occur they may be explained by factors common to many other types of assay.
有观点认为，酶放大免疫测定技术（EMIT）会产生虚假结果——即所谓的“异常值”，许多实验室因此对这类检测进行重复操作。在本研究中，使用Cobas - Bio离心分析仪以及作者实验室开发的经济高效的检测方法，针对三种抗癫痫药物的血浆样本以及五种滥用药物的尿液样本进行了完整的EMIT检测，每次检测都有各自的校准曲线。结果未获得严重的虚假结果。这表明EMIT检测本身不存在产生“异常值”的内在倾向，当出现此类情况时，或许可以用许多其他类型检测共有的因素来解释。
