Obruca A, Strohmer H, Sakkas D, Menezo Y, Kogosowski A, Barak Y, Feichtinger W
Institute of Sterility Treatment, Vienna, Austria.
Contracept Fertil Sex. 1994 May;22(5):303-5.
Erbium-YAG laser has been applied for micromanipulation in human beings. In a first series of attempts it was used in the fertilization process for subzonal insemination (SUZI): laser assisted micromanipulation achieved significant higher fertilization rates (34.8%) when compared to mechanical SUZI (16.1%), and for partial zona dissection (PZD): in this case laser did not improve the results (laser 14.8% vs mechanical 14%). Erbium-YAG laser was used to assist hatching. In the mouse it significantly improved the hatching rate (80% vs 29.3%) 110 hours post hCG. This technique was applied in two different centres to patients with previous IVF failures. The implantation rate per embryo (14.4% laser assisted hatching vs 6% control group) and the pregnancy rate per transfer (40% vs 16.2%) were improved.
铒钇铝石榴石激光已应用于人体的显微操作。在最初的一系列尝试中，它被用于受精过程中的透明带下授精（SUZI）：与机械SUZI（16.1%）相比，激光辅助显微操作实现了显著更高的受精率（34.8%）；以及部分透明带切割（PZD）：在这种情况下，激光并未改善结果（激光组为14.8%，机械组为14%）。铒钇铝石榴石激光被用于辅助孵化。在小鼠中，在注射人绒毛膜促性腺激素（hCG）110小时后，它显著提高了孵化率（80%对29.3%）。该技术在两个不同的中心应用于既往体外受精（IVF）失败的患者。每个胚胎的着床率（激光辅助孵化组为14.4%，对照组为6%）和每次移植的妊娠率（40%对16.2%）均得到了提高。
