Rosseel M T, Lefebvre R A
Heymans Institute of Pharmacology, University of Ghent Medical School, Belgium.
J Chromatogr A. 1994 May 13;668(2):475-80. doi: 10.1016/0021-9673(94)80142-8.
A capillary gas chromatographic method with nitrogen-phosphorus detection for the simultaneous determination of nicardipine and its pyridine metabolite M-5 was developed. The method involves extraction of the plasma with hexane-methylene chloride (1:1, v/v), followed by evaporation of the organic phase. The extract is injected into a fused-silica capillary column coated with cross-linked 5% phenyl-methylsilicone. A temperature gradient (85-285 degrees C) is applied and the two products and the internal standard can be separated within 22 min. The limit of detection is 0.5 ng/ml for both products. The method is suitable for pharmacokinetic studies in humans.
建立了一种采用氮磷检测的毛细管气相色谱法，用于同时测定尼卡地平及其吡啶代谢物M-5。该方法包括用正己烷 - 二氯甲烷（1:1，v/v）萃取血浆，然后蒸发有机相。将提取物注入涂有交联5%苯基 - 甲基硅氧烷的熔融石英毛细管柱。采用温度梯度（85 - 285℃），两种产物和内标物可在22分钟内分离。两种产物的检测限均为0.5 ng/ml。该方法适用于人体药代动力学研究。
