Rosseel M T, Bogaert M G
J Chromatogr. 1983 Nov 25;279:675-80. doi: 10.1016/s0021-9673(01)93672-6.
A gas chromatographic method for the determination of nifedipine in human plasma is presented. Nifedipine was extracted from plasma at basic pH with toluene, and nitrendipine was used as the internal standard. Chromatography was performed on a cross-linked methylsilicone fused-silica column by on-column injection and with a nitrogen-phosphorus ionization detector. The minimal detectable concentration was approximately equal to 0.5 ng/ml of plasma. The standard curve was linear in the range evaluated, 2-300 ng/ml plasma. The within-analysis coefficient of variation was 3.9-10.4%, and the day-to-day coefficient of variation was 3.8%. A peak with the same retention time as the nitropyridine derivative of nifedipine was detected in the plasma of patients who had taken 10 mg nifedipine orally.
介绍了一种测定人血浆中硝苯地平的气相色谱法。在碱性pH条件下，用甲苯从血浆中萃取硝苯地平，尼群地平用作内标。采用柱上进样，在交联甲基硅氧烷熔融石英柱上进行色谱分析，并使用氮磷电离检测器。最低检测浓度约等于0.5 ng/ml血浆。在所评估的2 - 300 ng/ml血浆范围内，标准曲线呈线性。批内变异系数为3.9 - 10.4%，日间变异系数为3.8%。在口服10 mg硝苯地平的患者血浆中检测到一个与硝苯地平硝基吡啶衍生物保留时间相同的峰。
