Hiramoto R N, Ghanta V K, Soong S J, Hiramoto N S
Department of Microbiology, University of Alabama at Birmingham 35294.
J Immunother Emphasis Tumor Immunol. 1994 Apr;15(3):202-11. doi: 10.1097/00002371-199404000-00006.
Studies were initiated to determine if it was possible to use a tumor-bearing animal's own spleen cells to impart resistance to its neoplasm. YC8 T-cell lymphoma-bearing BALB/c mice (TBAs) were immunized with allogeneic DBA/2 spleen cells, which share cross-reacting antigens with the YC8 tumor. Animals immunized with the alloantigen were splenectomized and their spleen cells co-cultured with additional alloantigens for 2 days in media containing 2% polyethylene glycol (PEG) before being returned to the cyclophosphamide (cytoxan) (Ctx) pretreated autologous host. Treatment with Ctx was used to reduce suppressor factors in the TBA. It was found that when cultured spleen cells were returned to the autologous TBA, much greater resistance was imparted to the host and, in many instances, regression of the YC8 tumor was observed.
开展了多项研究，以确定是否有可能利用荷瘤动物自身的脾细胞赋予其对肿瘤的抗性。用同种异体DBA/2脾细胞对携带YC8 T细胞淋巴瘤的BALB/c小鼠（TBA）进行免疫，DBA/2脾细胞与YC8肿瘤具有交叉反应抗原。用同种异体抗原免疫的动物进行脾切除，其脾细胞在含有2%聚乙二醇（PEG）的培养基中与额外的同种异体抗原共培养2天，然后再回输到经环磷酰胺（细胞毒素）（Ctx）预处理的自体宿主中。使用Ctx进行治疗是为了减少TBA中的抑制因子。结果发现，当将培养的脾细胞回输到自体TBA中时，宿主获得了更强的抗性，并且在许多情况下，观察到YC8肿瘤出现消退。
