Scacciati J M, Mancini R E
Fertil Steril. 1975 Jan;26(1):6-12. doi: 10.1016/s0015-0282(16)40869-1.
Human spermatozoa separated from normal semen were washed with NaC1 (0.9%)and then were washed nine and 20 times with water. Soluble and insoluble fractions were thus obtained. Proteins, nucleoproteins, lipids, and carbohydrates were determined in both fractions. Different subproducts were obtained from the insoluble fraction, and analysis of diffusible proteins was made by disc electrophoresis. They were also used as antigens in immunoserologic reactions against anti-human seminal plasma, antihuman serum proteins, and anti-insoluble fraction of sperm cells. Aglycopeptide, isolated from human seminal plasma, was also employed as antigen against the antisera. The following results were observed: (1) the soluble fraction showed a high concentration of proteins and carbohydrates and a negligible amount of nucleic acid; (2) the insoluble fraction (nine times washed spermatozoa) had a high content of proteins, low concentration of carbohydrates, and no lipids; (3) cross reactions were observed by immunodiffusion test among spermatozoa washed nine times, spermatozoa washed 20 times, seminal plasma and soluble fraction against antiseminal plasma and anti-insoluble fraction SRM9, suggesting the presence of similar antigenic groups; and (4) immunoelectrophoresis showed three arcs for nine times washed speratozoa against anti-insoluble fraction and anti-seminal plasma.
从正常精液中分离出的人类精子先用0.9%的氯化钠溶液洗涤，然后用水洗涤9次和20次。由此获得可溶部分和不溶部分。对这两部分都进行了蛋白质、核蛋白、脂质和碳水化合物的测定。从不溶部分获得了不同的副产物，并通过圆盘电泳对可扩散蛋白质进行了分析。它们还被用作免疫血清学反应中的抗原，以对抗抗人精浆、抗人血清蛋白和抗精子细胞不溶部分。从人精浆中分离出的糖肽也被用作抗血清的抗原。观察到以下结果：(1)可溶部分显示蛋白质和碳水化合物浓度高，核酸含量可忽略不计；(2)不溶部分（洗涤9次的精子）蛋白质含量高，碳水化合物浓度低，且无脂质；(3)通过免疫扩散试验观察到，洗涤9次的精子、洗涤20次的精子、精浆和可溶部分与抗精浆和抗不溶部分SRM9之间存在交叉反应，表明存在相似的抗原基团；(4)免疫电泳显示，洗涤9次的精子对抗不溶部分和抗精浆产生三条弧。
